Target：AF-4
Fields：Transcriptional misregulation in cancer
Gene Name：AFF1
Protein Name：AF4/FMR2 family member 1
Human Gene Id：4299
Human Swiss Prot No：P51825
Mouse Swiss Prot No：O88573
Immunogen：The antiserum was produced against synthesized peptide derived from human AF4. AA range:1-50
Specificity：AF-4 Polyclonal Antibody detects endogenous levels of AF-4 protein.
Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source：Polyclonal, Rabbit,IgG
Dilution：WB 1:500-2000 IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration：1 mg/ml
Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name：AFF1;AF4;FEL;MLLT2;PBM1;AF4/FMR2 family member 1;ALL1-fused gene from chromosome 4 protein;Protein AF-4;Protein FEL;Proto-oncogene AF4
Observed Band(KD)：130kD
Background： This gene encodes a member of the AF4/ lymphoid nuclear protein related to AF4/Fragile X E mental retardation syndrome family of proteins, which have been implicated in childhood lymphoblastic leukemia, Fragile X E site mental retardation, and ataxia. It is the prevalent mixed-lineage leukemia fusion gene associated with spontaneous acute lymphoblastic leukemia. Members of this family have three conserved domains: an N-terminal homology domain, an AF4/ lymphoid nuclear protein related to AF4/Fragile X E mental retardation syndrome domain, and a C-terminal homology domain. The protein functions as a regulator of RNA polymerase II-mediated transcription through elongation and chromatin remodeling functions. Through RNA interference screens, this gene has been shown to promote the expression of CD133, a plasma membrane glycoprotein required for leukemia cell survival. Alternative splicing results in mu
Function：disease:A chromosomal aberration involving AFF1 is associated with acute leukemias. Translocation t(4;11)(q21;q23) with MLL/HRX. The result is a rogue activator protein.,similarity:Belongs to the AF4 family.,
Subcellular Location：Nucleus .
Expression： Epithelium,PCR rescued clones,Placenta,
