Target：Nek7

Fields：NOD-like receptor signaling pathway

Gene Name：NEK7

Protein Name：Serine/threonine-protein kinase Nek7

Human Gene Id：140609

Human Swiss Prot No：Q8TDX7

Mouse Gene Id：59125

Mouse Swiss Prot No：Q9ES74

Immunogen：The antiserum was produced against synthesized peptide derived from human NEK7. AA range:101-150

Specificity：Nek7 Polyclonal Antibody detects endogenous levels of Nek7 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：NEK7;Serine/threonine-protein kinase Nek7;Never in mitosis A-related kinase 7;NimA-related protein kinase 7

Observed Band(KD)：35kD

Background： NIMA-related kinases share high amino acid sequence identity with the gene product of the Aspergillus nidulans 'never in mitosis A' gene, which controls initiation of mitosis.[supplied by OMIM, Jul 2002],

Function：catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.,similarity:Contains 1 protein kinase domain.,tissue specificity:Highly expressed in lung, muscle, testis, brain, heart, liver, leukocyte and spleen. Lower expression in ovary, prostate and kidney. No expression seen in small intestine.,

Subcellular Location：Nucleus . Cytoplasm . Cytoplasm, cytoskeleton, spindle pole . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Present at centrosome throughout the cell cycle (PubMed:17586473). Also detected at spindle midzone of the anaphase cells and eventually concentrates at the midbody (PubMed:17586473). Interaction with ANKS3 prevents its translocation to the nucleus (By similarity). .

Expression：Highly expressed in lung, muscle, testis, brain, heart, liver, leukocyte and spleen. Lower expression in ovary, prostate and kidney. No expression seen in small intestine.