Target：NEIL3

Fields：Base excision repair

Gene Name：NEIL3

Protein Name：Endonuclease 8-like 3

Human Gene Id：55247

Human Swiss Prot No：Q8TAT5

Mouse Gene Id：234258

Mouse Swiss Prot No：Q8K203

Immunogen：The antiserum was produced against synthesized peptide derived from human NEIL3. AA range:491-540

Specificity：NEIL3 Polyclonal Antibody detects endogenous levels of NEIL3 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：NEIL3;Endonuclease 8-like 3;DNA glycosylase FPG2;DNA glycosylase/AP lyase Neil3;Endonuclease VIII-like 3;Nei-like protein 3

Observed Band(KD)：68kD

Background： NEIL3 belongs to a class of DNA glycosylases homologous to the bacterial Fpg/Nei family. These glycosylases initiate the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break via the associated lyase reaction (Bandaru et al., 2002 [PubMed 12509226]).[supplied by OMIM, Mar 2008],

Function：function:Reports about DNA glycosylase activity are contradictory. The protein lacks a proline residue at the N-terminus that is the active site residue found in the other members of the FPG family.,similarity:Belongs to the FPG family.,similarity:Contains 1 FPG-type zinc finger.,similarity:Contains 1 RanBP2-type zinc finger.,tissue specificity:Detected in thymus and testis.,

Subcellular Location：Nucleus . Chromosome . Recruited to replication stress sites via interaction with ubiquitinated CMG helicase. .

Expression：Expressed in keratinocytes and embryonic fibroblasts (at protein level). Also detected in thymus, testis and fetal lung primary fibroblasts.