Target：ADAR1

Fields：Cytosolic DNA-sensing pathway;Measles;Influenza A;Coronavirus disease - COVID-19

Gene Name：ADAR

Protein Name：Double-stranded RNA-specific adenosine deaminase

Human Gene Id：103

Human Swiss Prot No：P55265

Mouse Gene Id：56417

Mouse Swiss Prot No：Q99MU3

Rat Gene Id：81635

Rat Swiss Prot No：P55266

Immunogen：The antiserum was produced against synthesized peptide derived from human ADAR1. AA range:1172-1221

Specificity：ADAR1 Polyclonal Antibody detects endogenous levels of ADAR1 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500-2000 IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：ADAR;ADAR1;DSRAD;G1P1;IFI4;Double-stranded RNA-specific adenosine deaminase;DRADA;136 kDa double-stranded RNA-binding protein;p136;Interferon-inducible protein 4;IFI-4;K88DSRBP

Observed Band(KD)：135kD

Background：adenosine deaminase, RNA specific(ADAR) Homo sapiens This gene encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double-stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2010],

Function：caution:The N-terminus of isoform 4 has been derived from EST and genomic sequences.,disease:Defects in ADAR are a cause of dyschromatosis symmetrical hereditaria (DSH) [MIM:127400]; also known as reticulate acropigmentation of Dohi. DSH is a pigmentary genodermatosis of autosomal dominant inheritance characterized by a mixture of hyperpigmented and hypopigmented macules distributed on the dorsal parts of the hands and feet.,function:Converts multiple adenosines to inosines and creates I/U mismatched base pairs in double-helical RNA substrates without apparent sequence specificity. Has been found to modify more frequently adenosines in AU-rich regions, probably due to the relative ease of melting A/U base pairs as compared to G/C pairs. Functions to modify viral RNA genomes and may be responsible for hypermutation of certain negative-stranded viruses. Edits the messenger RNAs for glutama

Subcellular Location：[Isoform 1]: Cytoplasm . Nucleus . Shuttles between the cytoplasm and nucleus (PubMed:7565688, PubMed:24753571). Nuclear import is mediated by TNPO1 (PubMed:24753571). .; [Isoform 5]: Cytoplasm . Nucleus . Nucleus, nucleolus . Predominantly nuclear but can shuttle between nucleus and cytoplasm. TNPO1 can mediate its nuclear import whereas XPO5 can mediate its nuclear export. .

Expression：Ubiquitously expressed, highest levels were found in brain and lung (PubMed:7972084). Isoform 5 is expressed at higher levels in astrocytomas as compared to normal brain tissue and expression increases strikingly with the severity of the tumor, being higher in the most aggressive tumors.