Target:mTOR
Fields:EGFR tyrosine kinase inhibitor resistance;Endocrine resistance;ErbB signaling pathway;HIF-1 signaling pathway;Phospholipase D signaling pathway;Autophagy - other;Autophagy - animal;mTOR signaling pathway;PI3K-Akt signaling pathway;AMPK signaling pathway;Longevity regulating pathway;Longevity regulating pathway - multiple species;Cellular senescence;Apelin signaling pathway;Neutrophil extracellular trap formation;JAK-STAT signaling pathway;Th17 cell differentiation;Thermogenesis;Insulin signaling pathway;Thyroid hormone signaling pathway;Adipocytokine signaling pathway;Type II diabetes mellitus;Insulin resistance;Growth hormone synthesis, secretion and action;Alzheimer disease;Amyotrophic lateral sclerosis;Huntington disease;Spinocerebellar ataxia;Pathways of neurodegeneration - multiple diseases;Shigellosis;Human cytomegalovirus infection;Human papillomavirus infection;Kaposi sarcoma-associated herpesvirus infection;He
Gene Name:MTOR
Protein Name:Serine/threonine-protein kinase mTOR
Human Gene Id:2475
Human Swiss Prot No:P42345
Mouse Gene Id:56717
Mouse Swiss Prot No:Q9JLN9
Rat Gene Id:56718
Rat Swiss Prot No:P42346
Immunogen:The antiserum was produced against synthesized peptide derived from human mTOR. AA range:2447-2496
Specificity:mTOR Polyclonal Antibody detects endogenous levels of mTOR protein.
Formulation:Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source:Polyclonal, Rabbit,IgG
Dilution:WB 1:500-2000;IHC 1:100-500;IF ICC 1:100-500;ELISA 1:5000-20000
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:1 mg/ml
Storage Stability:-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name:MTOR;FRAP;FRAP1;FRAP2;RAFT1;RAPT1;Serine/threonine-protein kinase mTOR;FK506-binding protein 12-rapamycin complex-associated protein 1;FKBP12-rapamycin complex-associated protein;Mammalian target of rapamycin;mTOR;Mechanistic tar
Observed Band(KD):289kD
Background: The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq, Sep 2008],
Function:function:Acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. Part of the TORC2 complex which plays a critical role in AKT1 Ser-473 phosphorylation, and may modulate the phosphorylation of PKCA and regulate actin cytoskeleton organization.,similarity:Belongs to the PI3/PI4-kinase family.,similarity:Contains 1 FAT domain.,similarity:Contains 1 FATC domain.,similarity:Contains 1 PI3K/PI4K domain.,similarity:Contains 7 HEAT repeats.,subunit:Interacts with the FKBP12-rapamycin complex. Binds UBQLN1. Forms part of the mammalian target of rapamycin 2 complex (TORC2) comprised of FRAP1, GBL, PRR5, RICTOR and SIN. TORC2 does not bind to and is not sensitive to FKBP12-rapamycin. Binds directly to PRR5 and RICTOR within the TORC2 complex.,tissue specificity:Expressed in numerous tissues, with highest levels in testis.,
Subcellular Location:Endoplasmic reticulum membrane ; Peripheral membrane protein ; Cytoplasmic side . Golgi apparatus membrane ; Peripheral membrane protein ; Cytoplasmic side . Mitochondrion outer membrane ; Peripheral membrane protein ; Cytoplasmic side . Lysosome . Cytoplasm . Nucleus, PML body . Microsome membrane . Lysosome membrane . Cytoplasmic vesicle, phagosome . Shuttles between cytoplasm and nucleus. Accumulates in the nucleus in response to hypoxia (By similarity). Targeting to lysosomes depends on amino acid availability and RRAGA and RRAGB (PubMed:18497260, PubMed:20381137). Lysosome targeting also depends on interaction with MEAK7. Translocates to the lysosome membrane in the presence of TM4SF5 (PubMed:30956113). .
Expression:Expressed in numerous tissues, with highest levels in testis.