Target:O-FucT-1
Fields:Other types of O-glycan biosynthesis
Gene Name:POFUT1
Protein Name:GDP-fucose protein O-fucosyltransferase 1
Human Gene Id:23509
Human Swiss Prot No:Q9H488
Mouse Gene Id:140484
Mouse Swiss Prot No:Q91ZW2
Rat Gene Id:311551
Rat Swiss Prot No:Q6EV70
Immunogen:The antiserum was produced against synthesized peptide derived from human POFUT1. AA range:331-380
Specificity:O-FucT-1 Polyclonal Antibody detects endogenous levels of O-FucT-1 protein.
Formulation:Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source:Polyclonal, Rabbit,IgG
Dilution:WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:1 mg/ml
Storage Stability:-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name:POFUT1;FUT12;KIAA0180;GDP-fucose protein O-fucosyltransferase 1;Peptide-O-fucosyltransferase 1;O-FucT-1
Observed Band(KD):44kD
Background: This gene encodes a member of the glycosyltransferase O-Fuc family. This enzyme adds O-fucose through an O-glycosidic linkage to conserved serine or threonine residues in the epidermal growth factor-like repeats of a number of cell surface and secreted proteins. O-fucose glycans are involved in ligand-induced receptor signaling. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008],
Function:catalytic activity:Transfers an alpha-L-fucosyl residue from GDP-beta-L-fucose to the serine hydroxy group of a protein acceptor.,cofactor:Manganese.,function:Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in EGF domains. Plays a crucial role in Notch signaling.,online information:GlycoGene database,online information:Peptide-O-fucosyltransferase 1,pathway:Protein modification; protein glycosylation.,similarity:Belongs to the glycosyltransferase 68 family.,tissue specificity:Highly expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.,
Subcellular Location:Endoplasmic reticulum .
Expression:Highly expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.