Target：PARP-4

Fields：Base excision repair;Apoptosis

Gene Name：PARP4

Protein Name：Poly [ADP-ribose] polymerase 4

Human Gene Id：143

Human Swiss Prot No：Q9UKK3

Immunogen：The antiserum was produced against synthesized peptide derived from human PARP4. AA range:1151-1200

Specificity：PARP-4 Polyclonal Antibody detects endogenous levels of PARP-4 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：PARP4;ADPRTL1;KIAA0177;PARPL;Poly [ADP-ribose] polymerase 4;PARP-4;193 kDa vault protein;ADP-ribosyltransferase diphtheria toxin-like 4;ARTD4;PARP-related/IalphaI-related H5/proline-rich;PH5P;Vault poly(ADP-ribose) polymerase;VP

Molecular Weight(Da)：193kD

Background： This gene encodes poly(ADP-ribosyl)transferase-like 1 protein, which is capable of catalyzing a poly(ADP-ribosyl)ation reaction. This protein has a catalytic domain which is homologous to that of poly (ADP-ribosyl) transferase, but lacks an N-terminal DNA binding domain which activates the C-terminal catalytic domain of poly (ADP-ribosyl) transferase. Since this protein is not capable of binding DNA directly, its transferase activity may be activated by other factors such as protein-protein interaction mediated by the extensive carboxyl terminus. [provided by RefSeq, Jul 2008],

Function：catalytic activity:NAD(+) + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor.,similarity:Contains 1 BRCT domain.,similarity:Contains 1 PARP alpha-helical domain.,similarity:Contains 1 PARP catalytic domain.,similarity:Contains 1 VWFA domain.,subcellular location:Also found in the nucleus, associated with mitotic spindles.,subunit:Component of the vault ribonucleoprotein particle, at least composed of MVP, PARP4 and one or more vault RNAs (vRNAs). Binds to MVP. Associates with TEP1.,tissue specificity:Widely expressed; the highest levels are in the kidney; also detected in heart, placenta, lung, liver, skeletal muscle, spleen, leukocytes and pancreas.,

Subcellular Location：Cytoplasm . Nucleus . Cytoplasm, cytoskeleton, spindle . Also found in the nucleus, associated with mitotic spindles. .

Expression：Widely expressed; the highest levels are in the kidney; also detected in heart, placenta, lung, liver, skeletal muscle, spleen, leukocytes and pancreas.