Target:PARP-2
Fields:Base excision repair;Apoptosis
Gene Name:PARP2
Protein Name:Poly [ADP-ribose] polymerase 2
Human Gene Id:10038
Human Swiss Prot No:Q9UGN5
Mouse Gene Id:11546
Mouse Swiss Prot No:O88554
Immunogen:The antiserum was produced against synthesized peptide derived from human PARP2. AA range:151-200
Specificity:PARP-2 Polyclonal Antibody detects endogenous levels of PARP-2 protein.
Formulation:Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source:Polyclonal, Rabbit,IgG
Dilution:WB 1:500 - 1:2000. ELISA: 1:40000. Not yet tested in other applications.
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:1 mg/ml
Storage Stability:-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name:PARP2;ADPRT2;ADPRTL2;Poly [ADP-ribose] polymerase 2;PARP-2;hPARP-2;ADP-ribosyltransferase diphtheria toxin-like 2;ARTD2;NAD(+) ADP-ribosyltransferase 2;ADPRT-2;Poly[ADP-ribose] synthase 2;pADPRT-2
Observed Band(KD):75kD
Background: This gene encodes poly(ADP-ribosyl)transferase-like 2 protein, which contains a catalytic domain and is capable of catalyzing a poly(ADP-ribosyl)ation reaction. This protein has a catalytic domain which is homologous to that of poly (ADP-ribosyl) transferase, but lacks an N-terminal DNA binding domain which activates the C-terminal catalytic domain of poly (ADP-ribosyl) transferase. The basic residues within the N-terminal region of this protein may bear potential DNA-binding properties, and may be involved in the nuclear and/or nucleolar targeting of the protein. Two alternatively spliced transcript variants encoding distinct isoforms have been found. [provided by RefSeq, Jul 2008],
Function:catalytic activity:NAD(+) + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor.,function:Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks.,PTM:Poly-ADP-ribosylated by PARP1.,similarity:Contains 1 PARP alpha-helical domain.,similarity:Contains 1 PARP catalytic domain.,subunit:Component of a base excision repair (BER) complex, containing at least XRCC1, PARP1, POLB and LIG3. Homo- and heterodimer with PARP1.,tissue specificity:Widely expressed, mainly in actively dividing tissues. The highest levels are in the brain, heart, pancreas, skeletal muscle and testis; also detected i
Subcellular Location:Nucleus . Chromosome . Recruited to DNA damage sites. .
Expression:Widely expressed, mainly in actively dividing tissues (PubMed:10364231). The highest levels are in the brain, heart, pancreas, skeletal muscle and testis; also detected in kidney, liver, lung, placenta, ovary and spleen; levels are low in leukocytes, colon, small intestine, prostate and thymus (PubMed:10364231).