Target:PC-PLD3
Fields:Glycerophospholipid metabolism;Ether lipid metabolism;Metabolic pathways
Gene Name:PLD3
Protein Name:Phospholipase D3
Human Gene Id:23646
Human Swiss Prot No:Q8IV08
Mouse Gene Id:18807
Mouse Swiss Prot No:O35405
Rat Gene Id:361527
Rat Swiss Prot No:Q5FVH2
Immunogen:The antiserum was produced against synthesized peptide derived from human PLD3. AA range:326-375
Specificity:PC-PLD3 Polyclonal Antibody detects endogenous levels of PC-PLD3 protein.
Formulation:Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source:Polyclonal, Rabbit,IgG
Dilution:IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:1 mg/ml
Storage Stability:-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name:PLD3;Phospholipase D3;PLD 3;Choline phosphatase 3;HindIII K4L homolog;Hu-K4;Phosphatidylcholine-hydrolyzing phospholipase D3
Molecular Weight(Da):55kD
Background: This gene encodes a member of the phospholipase D (PLD) family of enzymes that catalyze the hydrolysis of membrane phospholipids. The encoded protein is a single-pass type II membrane protein and contains two PLD phosphodiesterase domains. This protein influences processing of amyloid-beta precursor protein. Mutations in this gene are associated with Alzheimer disease risk. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Apr 2014],
Function:catalytic activity:A phosphatidylcholine + H(2)O = choline + a phosphatidate.,PTM:Glycosylated.,similarity:Belongs to the phospholipase D family.,similarity:Contains 2 PLD phosphodiesterase domains.,tissue specificity:Widely expressed. Expressec at higher level in brain. Expresse at low level in corpus callosum, suggsting that it is highly expressed in neurons.,
Subcellular Location:Endoplasmic reticulum membrane ; Single-pass type II membrane protein . Lysosome lumen . Early endosome membrane ; Single-pass type II membrane protein . Late endosome membrane ; Single-pass type II membrane protein . Golgi apparatus membrane ; Single-pass type II membrane protein . Endosome membrane ; Single-pass type II membrane protein . Localizes to ER-associated vesicles in differentiating myotubes (PubMed:22428023). The soluble form in lysosome arises by proteolytic processing of the membrane-bound form (PubMed:29386126). Colocalizes with APP in endosomes (PubMed:29368044). .
Expression:Widely expressed. In the brain, high levels of expression are detected in the frontal, temporal and occipital cortices and hippocampus. Expressed at low level in corpus callosum.