Target:PFK-B
Fields:Glycolysis / Gluconeogenesis;Pentose phosphate pathway;Fructose and mannose metabolism;Galactose metabolism;Metabolic pathways;Carbon metabolism;Biosynthesis of amino acids;RNA degradation;HIF-1 signaling pathway;AMPK signaling pathway;Thyroid hormone signaling pathway;Glucagon signaling pathway;Central carbon metabolism in cancer
Gene Name:PFKL
Protein Name:6-phosphofructokinase liver type
Human Gene Id:5211
Human Swiss Prot No:P17858
Mouse Gene Id:18641
Mouse Swiss Prot No:P12382
Rat Gene Id:25741
Rat Swiss Prot No:P30835
Immunogen:The antiserum was produced against synthesized peptide derived from human K6PL. AA range:691-740
Specificity:PFK-B Polyclonal Antibody detects endogenous levels of PFK-B protein.
Formulation:Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source:Polyclonal, Rabbit,IgG
Dilution:WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:1 mg/ml
Storage Stability:-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name:PFKL;6-phosphofructokinase; liver type;Phosphofructo-1-kinase isozyme B;PFK-B;Phosphofructokinase 1;Phosphohexokinase
Observed Band(KD):85kD
Background: This gene encodes the liver (L) subunit of an enzyme that catalyzes the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate, which is a key step in glucose metabolism (glycolysis). This enzyme is a tetramer that may be composed of different subunits encoded by distinct genes in different tissues. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014],
Function:catalytic activity:ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.,cofactor:Magnesium.,enzyme regulation:Allosteric enzyme activated by ADP, AMP, or fructose bisphosphate and inhibited by ATP or citrate.,miscellaneous:In human PFK exists as a system of 3 types of subunits, PFKM (muscle), PFKL (liver) and PFKP (platelet) isoenzymes.,pathway:Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.,similarity:Belongs to the phosphofructokinase family. Two domains subfamily.,subunit:Tetramer. Muscle is M4, liver is L4, and red cell is M3L, M2L2, or ML3.,
Subcellular Location:Cytoplasm .
Expression: Brain,Cervix,Colon,Human amygdala,Human retina,Kidney,Liver,Lung,Muscle,Pla