Target：PI 3-kinase p101

Fields：cGMP-PKG signaling pathway;Chemokine signaling pathway;Phospholipase D signaling pathway;PI3K-Akt signaling pathway;Adrenergic signaling in cardiomyocytes;Apelin signaling pathway;Platelet activation;Cholinergic synapse;Oxytocin signaling pathway;Toxoplasmosis;Kaposi sarcoma-associated herpesvirus infection

Gene Name：PIK3R5

Protein Name：Phosphoinositide 3-kinase regulatory subunit 5

Human Gene Id：23533

Human Swiss Prot No：Q8WYR1

Mouse Gene Id：320207

Mouse Swiss Prot No：Q5SW28

Immunogen：The antiserum was produced against synthesized peptide derived from human PIK3R5. AA range:695-744

Specificity：PI 3-kinase p101 Polyclonal Antibody detects endogenous levels of PI 3-kinase p101 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：PIK3R5;Phosphoinositide 3-kinase regulatory subunit 5;PI3-kinase regulatory subunit 5;PI3-kinase p101 subunit;Phosphatidylinositol 4;5-bisphosphate 3-kinase regulatory subunit;PtdIns-3-kinase regulatory subunit;Protein FOAP-2;PtdIns-

Observed Band(KD)：100kD

Background： Phosphatidylinositol 3-kinases (PI3Ks) phosphorylate the inositol ring of phosphatidylinositol at the 3-prime position, and play important roles in cell growth, proliferation, differentiation, motility, survival and intracellular trafficking. The PI3Ks are divided into three classes: I, II and III, and only the class I PI3Ks are involved in oncogenesis. This gene encodes the 101 kD regulatory subunit of the class I PI3K gamma complex, which is a dimeric enzyme, consisting of a 110 kD catalytic subunit gamma and a regulatory subunit of either 55, 87 or 101 kD. This protein recruits the catalytic subunit from the cytosol to the plasma membrane through high-affinity interaction with G-beta-gamma proteins. Multiple alternatively spliced transcript variants encoding two distinct isoforms have been found. [provided by RefSeq, Oct 2011],

Function：domain:The heterodimerization region allows the binding to the catalytic subunit.,enzyme regulation:Greatly activated by G gamma proteins.,function:Regulatory subunit of the PI3K gamma complex.,subunit:Heterodimer of a catalytic subunit (PIK3CG/p120) and a regulatory (PIK3R5a/p101) subunit. Interacts with G beta gamma proteins.,tissue specificity:Highly expressed in leukocytes, followed by spleen, lymph node, thymus ans bone marrow.,

Subcellular Location：Nucleus . Cytoplasm . Cell membrane ; Peripheral membrane protein . Predominantly localized in the nucleus in absence of PIK3CG/p120. Colocalizes with PIK3CG/p120 in the cytoplasm. Translocated to the plasma membrane in a beta-gamma G protein-dependent manner. .

Expression：Ubiquitously expressed with high expression in fetal brain compared to adult brain. Abundant expression is observed in cerebellum, cerebral cortex, cerebral meninges, and vermis cerebelli.