Target:Rab 5A
Fields:Ras signaling pathway;Endocytosis;Phagosome;Vasopressin-regulated water reabsorption;Amyotrophic lateral sclerosis;Pathways of neurodegeneration - multiple diseases;Salmonella infection;Amoebiasis;Tuberculosis
Gene Name:RAB5A
Protein Name:Ras-related protein Rab-5A
Human Gene Id:5868
Human Swiss Prot No:P20339
Mouse Gene Id:271457
Mouse Swiss Prot No:Q9CQD1
Immunogen:The antiserum was produced against synthesized peptide derived from the Internal region of human RAB5A. AA range:71-120
Specificity:Rab 5A Polyclonal Antibody detects endogenous levels of Rab 5A protein.
Formulation:Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source:Polyclonal, Rabbit,IgG
Dilution:WB 1:500 - 1:2000. IHC: 1:100-1:300. ELISA: 1:20000.. IF 1:50-200
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:1 mg/ml
Storage Stability:-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name:RAB5A;RAB5;Ras-related protein Rab-5A
Observed Band(KD):24kD
Background:enzyme regulation:Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP.,function:Required for the fusion of plasma membranes and early endosomes.,similarity:Belongs to the small GTPase superfamily. Rab family.,subcellular location:Enriched in stage I melanosomes.,subunit:Binds EEA1. Interacts with RIN1 and GAPVD1, which regulate its pathway, probably by acting as a GEF. Interacts with ALS2CL, UNC84B, ZFYVE20 and RUFY1. Interacts with SGSM1 and SGSM3.,
Function:enzyme regulation:Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP.,function:Required for the fusion of plasma membranes and early endosomes.,similarity:Belongs to the small GTPase superfamily. Rab family.,subcellular location:Enriched in stage I melanosomes.,subunit:Binds EEA1. Interacts with RIN1 and GAPVD1, which regulate its pathway, probably by acting as a GEF. Interacts with ALS2CL, UNC84B, ZFYVE20 and RUFY1. Interacts with SGSM1 and SGSM3.,
Subcellular Location:Cell membrane ; Lipid-anchor ; Cytoplasmic side . Early endosome membrane ; Lipid-anchor . Melanosome . Cytoplasmic vesicle . Cell projection, ruffle . Membrane . Cytoplasm, cytosol . Cytoplasmic vesicle, phagosome membrane . Endosome membrane . Enriched in stage I melanosomes (PubMed:17081065). Alternates between membrane-bound and cytosolic forms (Probable). .
Expression: Brain,Cervix,Cervix carcinoma,Placenta,