Target:Frizzled-7
Fields:mTOR signaling pathway;Wnt signaling pathway;Hippo signaling pathway;Signaling pathways regulating pluripotency of stem cells;Melanogenesis;Cushing syndrome;Alzheimer disease;Pathways of neurodegeneration - multiple diseases;Human papillomavirus infection;Pathways in cancer;Proteoglycans in cancer;Basal cell carcinoma;Breast cancer;Hepatocellular carcinoma;Gastric cancer
Gene Name:FZD7
Protein Name:Frizzled-7
Human Gene Id:8324
Human Swiss Prot No:O75084
Mouse Gene Id:14369
Mouse Swiss Prot No:Q61090
Immunogen:The antiserum was produced against synthesized peptide derived from human FZD7. AA range:45-94
Specificity:Frizzled-7 Polyclonal Antibody detects endogenous levels of Frizzled-7 protein.
Formulation:Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source:Polyclonal, Rabbit,IgG
Dilution:WB 1:500-2000;Flow Cyt 1:50-200;IF ICC 1:100-500;ELISA 1:5000-20000
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:1 mg/ml
Storage Stability:-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name:FZD7;Frizzled-7;Fz-7;hFz7;FzE3
Observed Band(KD):64kD
Background:frizzled class receptor 7(FZD7) Homo sapiens Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD7 protein contains an N-terminal signal sequence, 10 cysteine residues typical of the cysteine-rich extracellular domain of Fz family members, 7 putative transmembrane domains, and an intracellular C-terminal tail with a PDZ domain-binding motif. FZD7 gene expression may downregulate APC function and enhance beta-catenin-mediated signals in poorly differentiated human esophageal carcinomas. [provided by RefSeq, Jul 2008],
Function:domain:Lys-Thr-X-X-X-Trp motif is involved in the activation of the Wnt/beta-catenin signaling pathway.,domain:The FZ domain is involved in binding with Wnt ligands.,function:Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphog
Subcellular Location:Cell membrane ; Multi-pass membrane protein . Endosome membrane ; Multi-pass membrane protein . Associated to the plasma membrane in the presence of FZD7 and phosphatidylinositol 4,5-bisphosphate (PIP2). Localized in recycling endosomes in other conditions. .
Expression:High expression in adult skeletal muscle and fetal kidney, followed by fetal lung, adult heart, brain, and placenta. Specifically expressed in squamous cell esophageal carcinomas.