Target:Frizzled-5/8
Fields:mTOR signaling pathway;Wnt signaling pathway;Hippo signaling pathway;Signaling pathways regulating pluripotency of stem cells;Melanogenesis;Cushing syndrome;Alzheimer disease;Pathways of neurodegeneration - multiple diseases;Human papillomavirus infection;Pathways in cancer;Proteoglycans in cancer;Basal cell carcinoma;Breast cancer;Hepatocellular carcinoma;Gastric cancer
Gene Name:FZD5/FZD8
Protein Name:Frizzled-5/8
Human Gene Id:8325/7855
Human Swiss Prot No:Q13467/Q9H461
Mouse Gene Id:14367/14370
Rat Gene Id:317674/100909849
Rat Swiss Prot No:Q8CHL0/Q498S8
Immunogen:The antiserum was produced against synthesized peptide derived from human FZD8. AA range:31-80
Specificity:Frizzled-5/8 Polyclonal Antibody detects endogenous levels of Frizzled-5/8 protein.
Formulation:Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source:Polyclonal, Rabbit,IgG
Dilution:WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:1 mg/ml
Storage Stability:-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name:FZD5;C2orf31;Frizzled-5;Fz-5;hFz5;FzE5;FZD8;Frizzled-8;Fz-8;hFz8
Observed Band(KD):75kD
Background:frizzled class receptor 5(FZD5) Homo sapiens Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD5 protein is believed to be the receptor for the Wnt5A ligand. [provided by RefSeq, Jul 2008],
Function:domain:Lys-Thr-X-X-X-Trp motif is involved in the activation of the Wnt/beta-catenin signaling pathway.,domain:The FZ domain is involved in binding with Wnt ligands.,domain:The PDZ-binding motif mediates interaction with GOPC.,function:Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellul
Subcellular Location:Cell membrane ; Multi-pass membrane protein . Golgi apparatus membrane ; Multi-pass membrane protein . Cell junction, synapse . Perikaryon . Cell projection, dendrite . Cell projection, axon . Localized at the plasma membrane and also found at the Golgi. .
Expression: Oesophageal carcinoma,Retina,