Target：RECK

Fields：MicroRNAs in cancer

Gene Name：RECK

Protein Name：Reversion-inducing cysteine-rich protein with Kazal motifs

Human Gene Id：8434

Human Swiss Prot No：O95980

Mouse Gene Id：53614

Mouse Swiss Prot No：Q9Z0J1

Immunogen：The antiserum was produced against synthesized peptide derived from human RECK. AA range:21-70

Specificity：RECK Polyclonal Antibody detects endogenous levels of RECK protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500-2000 IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：RECK;ST15;Reversion-inducing cysteine-rich protein with Kazal motifs;hRECK;Suppressor of tumorigenicity 15 protein

Observed Band(KD)：110kD

Background： The protein encoded by this gene is a cysteine-rich, extracellular protein with protease inhibitor-like domains whose expression is suppressed strongly in many tumors and cells transformed by various kinds of oncogenes. In normal cells, this membrane-anchored glycoprotein may serve as a negative regulator for matrix metalloproteinase-9, a key enzyme involved in tumor invasion and metastasis. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015],

Function：function:Negatively regulates matrix metalloproteinase-9 (MMP-9) by suppressing MMP-9 secretion and by direct inhibition of its enzymatic activity. RECK down-regulation by oncogenic signals may facilitate tumor invasion and metastasis. Appears to also regulate MMP-2 and MT1-MMP, which are involved in cancer progression.,PTM:N-glycosylated.,similarity:Contains 3 Kazal-like domains.,subunit:Interacts with MMP-9.,tissue specificity:Expressed in various tissues and untransformed cells. It is undetectable in tumor-derived cell lines and oncogenically transformed cells.,

Subcellular Location：Cell membrane ; Lipid-anchor, GPI-anchor .

Expression：Expressed in various tissues and untransformed cells (PubMed:9789069). It is undetectable in tumor-derived cell lines and oncogenically transformed cells (PubMed:9789069).