Target：TFPI

Fields：Complement and coagulation cascades

Gene Name：TFPI

Protein Name：Tissue factor pathway inhibitor

Human Gene Id：7035

Human Swiss Prot No：P10646

Mouse Swiss Prot No：O54819

Immunogen：The antiserum was produced against synthesized peptide derived from the C-terminal region of human TFPI. AA range:251-300

Specificity：TFPI Polyclonal Antibody detects endogenous levels of TFPI protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. ELISA: 1:20000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：TFPI;LACI;TFPI1;Tissue factor pathway inhibitor;TFPI;Extrinsic pathway inhibitor;EPI;Lipoprotein-associated coagulation inhibitor;LACI

Observed Band(KD)：34kD

Background： This gene encodes a Kunitz-type serine protease inhibitor that regulates the tissue factor (TF)-dependent pathway of blood coagulation. The coagulation process initiates with the formation of a factor VIIa-TF complex, which proteolytically activates additional proteases (factors IX and X) and ultimately leads to the formation of a fibrin clot. The product of this gene inhibits the activated factor X and VIIa-TF proteases in an autoregulatory loop. Inhibition of the encoded protein restores hemostasis in animal models of hemophilia. This gene encodes multiple protein isoforms that differ in their inhibitory activity, specificity and cellular localization. [provided by RefSeq, Jul 2016],

Function：domain:This inhibitor contains three inhibitory domains. The first domain interacts with VIIa and TF, the second one with Xa.,function:Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma.,online information:TFPI entry,PTM:O-glycosylated.,similarity:Contains 2 BPTI/Kunitz inhibitor domains.,similarity:Contains 3 BPTI/Kunitz inhibitor domains.,tissue specificity:Mostly in endothelial cells.,

Subcellular Location：[Isoform Alpha]: Secreted.; [Isoform Beta]: Microsome membrane ; Lipid-anchor, GPI-anchor .

Expression：Mostly in endothelial cells.