Target：TLK2

Gene Name：TLK2

Protein Name：Serine/threonine-protein kinase tousled-like 2

Human Gene Id：11011

Human Swiss Prot No：Q86UE8

Mouse Gene Id：24086

Mouse Swiss Prot No：O55047

Immunogen：The antiserum was produced against synthesized peptide derived from human TLK2. AA range:191-240

Specificity：TLK2 Polyclonal Antibody detects endogenous levels of TLK2 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500-2000;IHC 1:50-300; ELISA 2000-20000

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：TLK2;Serine/threonine-protein kinase tousled-like 2;HsHPK;PKU-alpha;Tousled-like kinase 2

Observed Band(KD)：90kD

Background： This gene encodes a nuclear serine/threonine kinase that was first identified in Arabidopsis. The encoded protein is thought to function in the regulation of chromatin assembly in the S phase of the cell cycle by regulating the levels of a histone H3/H4 chaperone. This protein is associated with double-strand break repair of DNA damage caused by radiation. Pseudogenes of this gene are present on chromosomes 10 and 17. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013],

Function：catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Cell-cycle regulated, maximal activity in S-phase. Inactivated by phosphorylation at Ser-750, potentially by CHK1.,function:Rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase. This is cell cycle checkpoint and ATM-pathway dependent and appears to regulate processes involved in chromatin assembly.,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.,similarity:Contains 1 protein kinase domain.,subunit:Heterodimerizes with TLK1. Interacts with ASF1A and ASF1B.,tissue specificity:Widely expressed. Present in fetal placenta, liver, kidney, pancreas, heart and skeletal muscle. Also found in adult cell lines.,

Subcellular Location：Nucleus. Cytoplasm, perinuclear region. Cytoplasm, cytoskeleton. Colocalizes with the cytoplasmic intermediate filament system during the G1 phase of the cell cycle. Present in the perinuclear region at S phase and in the nucleus at late G2.

Expression：Ubiquitous. Detected in placenta, fetal liver, kidney, pancreas, heart and skeletal muscle. Highly expressed in testis. Detected in spleen, thymus, colon, ovary, small intestine, prostate and peripheral blood leukocytes.