Target：TIF1α

Gene Name：TRIM24

Protein Name：Transcription intermediary factor 1-alpha

Human Gene Id：8805

Human Swiss Prot No：O15164

Mouse Gene Id：21848

Mouse Swiss Prot No：Q64127

Immunogen：The antiserum was produced against synthesized peptide derived from human TRIM24. AA range:1001-1050

Specificity：TIF1α Polyclonal Antibody detects endogenous levels of TIF1α protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：TRIM24;RNF82;TIF1;TIF1A;Transcription intermediary factor 1-alpha;TIF1-alpha;E3 ubiquitin-protein ligase TRIM24;RING finger protein 82;Tripartite motif-containing protein 24

Observed Band(KD)：117kD

Background： The protein encoded by this gene mediates transcriptional control by interaction with the activation function 2 (AF2) region of several nuclear receptors, including the estrogen, retinoic acid, and vitamin D3 receptors. The protein localizes to nuclear bodies and is thought to associate with chromatin and heterochromatin-associated factors. The protein is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains - a RING, a B-box type 1 and a B-box type 2 - and a coiled-coil region. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008],

Function：disease:A chromosomal aberration involving TIF1 is a cause of thyroid papillary carcinoma (PACT) [MIM:188550]. Translocation t(7;10)(q32;q11) with RET. The translocation generates the TIF1/RET (PTC6) oncogene.,function:Interacts selectively in vitro with the AF2-activating domain of the estrogen receptors. Association with DNA-bound estrogen receptors requires the presence of estradiol.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Contains 1 bromo domain.,similarity:Contains 1 PHD-type zinc finger.,similarity:Contains 1 RING-type zinc finger.,similarity:Contains 2 B box-type zinc fingers.,subunit:Interacts with CBX1 and CBX3 (By similarity). Interacts with NR3C2.,

Subcellular Location：Nucleus . Cytoplasm . Mitochondrion . Colocalizes with sites of active transcription. Detected both in nucleus and cytoplasm in some breast cancer samples. Predominantly nuclear. Translocated from nucleus to mitochondria to mediate antiviral immunity (PubMed:32324863). .

Expression： Brain,Epithelium,Mammary cancer,Testis,Thyroid,