Target:TyrRS
Fields:Aminoacyl-tRNA biosynthesis
Gene Name:YARS
Protein Name:TyrRS
Human Gene Id:8565
Human Swiss Prot No:P54577
Mouse Gene Id:107271
Mouse Swiss Prot No:Q91WQ3
Rat Swiss Prot No:Q4KM49
Immunogen:The antiserum was produced against synthesized peptide derived from the C-terminal region of human YARS. AA range:451-500
Specificity:TyrRS Polyclonal Antibody detects endogenous levels of TyrRS
Formulation:Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source:Polyclonal, Rabbit,IgG
Dilution:WB 1:500-2000,IHC 1:50-300 ELISA 1:10000-20000
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:1 mg/ml
Storage Stability:-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name:Tyrosine--tRNA ligase, cytoplasmic (EC 6.1.1.1) (Tyrosyl-tRNA synthetase) (TyrRS)
Observed Band(KD):60kD
Background: Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Tyrosyl-tRNA synthetase belongs to the class I tRNA synthetase family. Cytokine activities have also been observed for the human tyrosyl-tRNA synthetase, after it is split into two parts, an N-terminal fragment that harbors the catalytic site and a C-terminal fragment found only in the mammalian enzyme. The N-terminal fragment is an interleukin-8-like cytokine, whereas the released C-terminal fragment is an EMAP II-like cytokine. [provided by RefSeq, Jul 2008],
Function:catalytic activity:ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr).,disease:Defects in YARS are the cause of Charcot-Marie-Tooth disease dominant intermediate type C (CMTDIC) [MIM:608323]. CMTDIC is a form of Charcot-Marie-Tooth disease characterized by clinical and pathologic features intermediate between demyelinating and axonal peripheral neuropathies, and motor median nerve conduction velocities ranging from 25 to 45 m/sec.,function:Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).,similarity:Belongs to the class-I aminoacyl-tRNA synthetase family.,similarity:Contains 1 tRNA-binding domain.,subunit:Homodimer.,
Subcellular Location:Cytoplasm .
Expression: B-cell lymphoma,Bone,Lung,Platelet,