Target:TOP1M
Gene Name:TOP1MT
Protein Name:DNA topoisomerase I, mitochondrial (TOP1mt) (EC 5.99.1.2)
Human Gene Id:116447
Human Swiss Prot No:Q969P6
Mouse Swiss Prot No:Q8R4U6
Rat Swiss Prot No:Q6IM78
Immunogen:Synthesized peptide derived from part region of human protein
Specificity:TOP1M Polyclonal Antibody detects endogenous levels of protein.
Formulation:Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
Source:Polyclonal, Rabbit,IgG
Dilution:WB 1:500-2000 ELISA 1:5000-20000
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:1 mg/ml
Storage Stability:-15°C to -25°C/1 year(Do not lower than -25°C)
Observed Band(KD):66kD
Background: This gene encodes a mitochondrial DNA topoisomerase that plays a role in the modification of DNA topology. The encoded protein is a type IB topoisomerase and catalyzes the transient breaking and rejoining of DNA to relieve tension and DNA supercoiling generated in the mitochondrial genome during replication and transcription. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012],
Function:catalytic activity:ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.,cofactor:Divalent metal ions (calcium or magnesium).,function:Relieves DNA strain that arise during duplication of mitochondrial DNA.,miscellaneous:When a topoisomerase transiently breaks a DNA backbone bond, it simultaneously forms a protein-DNA link, in which a tyrosyl oxygen in the enzyme is joined to a DNA phosphorus at one end of the enzyme-severed DNA strand.,similarity:Belongs to the eukaryotic type I topoisomerase family.,tissue specificity:Ubiquitous; highest in skeletal muscle, heart, brain and fetal liver.,
Subcellular Location:Mitochondrion .
Expression:Ubiquitous; highest in skeletal muscle, heart, brain and fetal liver.