Target：V-ATPase H

Fields：Oxidative phosphorylation;Metabolic pathways;Lysosome;Phagosome;mTOR signaling pathway;Synaptic vesicle cycle;Vibrio cholerae infection;Epithelial cell signaling in Helicobacter pylori infection;Tuberculosis;Human papillomavirus infection;Rheumatoid arthritis

Gene Name：ATP6V1H

Protein Name：V-type proton ATPase subunit H

Human Gene Id：51606

Human Swiss Prot No：Q9UI12

Mouse Gene Id：108664

Mouse Swiss Prot No：Q8BVE3

Immunogen：The antiserum was produced against synthesized peptide derived from human ATP6V1H. AA range:341-390

Specificity：V-ATPase H Polyclonal Antibody detects endogenous levels of V-ATPase H protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：ATP6V1H;CGI-11;V-type proton ATPase subunit H;V-ATPase subunit H;Nef-binding protein 1;NBP1;Protein VMA13 homolog;V-ATPase 50/57 kDa subunits;Vacuolar proton pump subunit H;Vacuolar proton pump subunit SFD

Observed Band(KD)：55kD

Background： This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular organelles. V-ATPase-dependent organelle acidification is necessary for multiple processes including protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. The encoded protein is the regulatory H subunit of the V1 domain of V-ATPase, which is required for catalysis of ATP but not the assembly of V-ATPase. Decreased expression of this gene may play a role in the development of type 2 diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012],

Function：function:Subunit of the peripheral V1 complex of vacuolar ATPase. Subunit H activates the ATPase activity of the enzyme and couples ATPase activity to proton flow. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system (By similarity). Involved in the endocytosis mediated by clathrin-coated pits, required for the formation of endosomes.,similarity:Belongs to the V-ATPase H subunit family.,subunit:V-ATPase is an heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'' and d). Interacts with HIV-1 Nef protein and AP2M1.,tissue specificity:Widely expressed.,

Subcellular Location：Cytoplasmic vesicle, clathrin-coated vesicle membrane ; Peripheral membrane protein .

Expression：Widely expressed.