Target：Tsg 101

Fields：Viral life cycle - HIV-1;Endocytosis

Gene Name：TSG101

Protein Name：Tumor susceptibility gene 101 protein

Human Gene Id：7251

Human Swiss Prot No：Q99816

Mouse Gene Id：22088

Mouse Swiss Prot No：Q61187

Rat Gene Id：292925

Rat Swiss Prot No：Q6IRE4

Immunogen：The antiserum was produced against synthesized peptide derived from human TSG101. AA range:281-330

Specificity：Tsg 101 Polyclonal Antibody detects endogenous levels of Tsg 101 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：TSG101;Tumor susceptibility gene 101 protein;ESCRT-I complex subunit TSG101

Observed Band(KD)：45kD

Background： The protein encoded by this gene belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. The gene product contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. The protein may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of this tumor susceptibility gene appears to be important for maintenance of genomic stability and cell cycle regulation. Mutations and alternative splicing in this gene occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression. [provided by RefSeq, Jul 2008],

Function：alternative products:Additional isoforms seem to exist. Several shorter isoforms are detected in primary breast cancers and other tumors,domain:The coiled coil domain may interact with stathmin.,domain:The UEV domain binds ubiquitin and P-[ST]-A-P peptide motif independently.,domain:The UEV domain is required for the interaction of the complex with ubiquitin. It also mediates the interaction with PTAP/PSAP motifs of HIV-1 P6 protein and human spumaretrovirus Gag protein.,function:Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a

Subcellular Location：Cytoplasm . Early endosome membrane ; Peripheral membrane protein ; Cytoplasmic side . Late endosome membrane ; Peripheral membrane protein. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Midbody, Midbody ring . Nucleus . Mainly cytoplasmic. Membrane-associated when active and soluble when inactive. Nuclear localization is cell cycle-dependent. Interaction with CEP55 is required for localization to the midbody during cytokinesis. .

Expression：Heart, brain, placenta, lung, liver, skeletal, kidney and pancreas.