Target：WNK2

Gene Name：WNK2 KIAA1760 PRKWNK2 SDCCAG43 P/OKcl.13

Protein Name：Serine/threonine-protein kinase WNK2 (EC 2.7.11.1) (Antigen NY-CO-43) (Protein kinase lysine-deficient 2) (Protein kinase with no lysine 2) (Serologically defined colon cancer antigen 43)

Human Gene Id：65268

Human Swiss Prot No：Q9Y3S1

Mouse Swiss Prot No：Q3UH66

Immunogen：Synthesized peptide derived from human protein . at AA range: 1120-1200

Specificity：WNK2 Polyclonal Antibody detects endogenous levels of protein.

Formulation：Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：IHC 1:50-300. IF 1:50-200

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Observed Band(KD)：252kD

Background： The protein encoded by this gene is a cytoplasmic serine-threonine kinase that belongs to the protein kinase superfamily. The protein plays an important role in the regulation of electrolyte homeostasis, cell signaling survival, and proliferation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013],

Function：catalytic activity:ATP + a protein = ADP + a phosphoprotein.,caution:Cys-224 is present instead of the conserved Lys which is expected to be an active site residue. Lys-207 appears to fulfill the required catalytic function.,cofactor:Magnesium.,enzyme regulation:Activation requires autophosphorylation of Ser-356. Phosphorylation of Ser-352 also promotes increased activity.,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily.,similarity:Contains 1 protein kinase domain.,tissue specificity:Predominantly expressed in heart, brain and colon.,

Subcellular Location：Cytoplasm . Cell membrane .

Expression：Expressed in various cancer cell lines (at protein level). Predominantly expressed in heart, brain, skeletal muscle and colon.