Target：TRAP100

Fields：Thyroid hormone signaling pathway

Gene Name：MED24

Protein Name：Mediator of RNA polymerase II transcription subunit 24

Human Gene Id：9862

Human Swiss Prot No：O75448

Mouse Gene Id：23989

Mouse Swiss Prot No：Q99K74

Rat Gene Id：619436

Rat Swiss Prot No：Q4V8B3

Immunogen：The antiserum was produced against synthesized peptide derived from human MED24. AA range:801-850

Specificity：TRAP100 Polyclonal Antibody detects endogenous levels of TRAP100 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：MED24;ARC100;CRSP4;DRIP100;KIAA0130;THRAP4;TRAP100;Mediator of RNA polymerase II transcription subunit 24;Activator-recruited cofactor 100 kDa component;ARC100;Cofactor required for Sp1 transcriptional activation subunit 4;CRSP c

Observed Band(KD)：110kD

Background： This gene encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],

Function：function:Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.,similarity:Belongs to the Mediator complex subunit 24 family.,subunit:Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 su

Subcellular Location：Nucleus .

Expression：Ubiquitous. Abundant in skeletal muscle, heart and placenta.