Target:GM2/GD2 synthase
Fields:Sphingolipid metabolism;Glycosphingolipid biosynthesis - ganglio series;Metabolic pathways
Gene Name:B4GALNT1
Protein Name:Beta-1,4 N-acetylgalactosaminyltransferase 1
Human Gene Id:2583
Human Swiss Prot No:Q00973
Mouse Gene Id:14421
Mouse Swiss Prot No:Q09200
Rat Gene Id:64828
Rat Swiss Prot No:Q10468
Immunogen:The antiserum was produced against synthesized peptide derived from human B4GALNT1. AA range:281-330
Specificity:GM2/GD2 synthase Polyclonal Antibody detects endogenous levels of GM2/GD2 synthase protein.
Formulation:Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source:Polyclonal, Rabbit,IgG
Dilution:WB 1:500 - 1:2000. ELISA: 1:5000. Not yet tested in other applications.
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:1 mg/ml
Storage Stability:-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name:B4GALNT1;GALGT;SIAT2;Beta-1;4 N-acetylgalactosaminyltransferase 1;(N-acetylneuraminyl)-galactosylglucosylceramide;GM2/GD2 synthase;GalNAc-T
Observed Band(KD):60kD
Background:beta-1,4-N-acetyl-galactosaminyltransferase 1(B4GALNT1) Homo sapiens GM2 and GD2 gangliosides are sialic acid-containing glycosphingolipids. GalNAc-T is the enzyme involved in the biosynthesis of G(M2) and G(D2) glycosphingolipids. GalNAc-T catalyzes the transfer of GalNAc into G(M3) and G(D3) by a beta-1,4 linkage, resulting in the synthesis of G(M2) and G(D2), respectively. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2013],
Function:catalytic activity:UDP-N-acetyl-D-galactosamine + 1-O-(O-(N-acetyl-alpha-neuraminosyl)-(2->3)-O-beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranosyl)-ceramide = UDP + 1-O-(O-2-(acetylamino)-2-deoxy-beta-D-galactopyranosyl-(1->4)-O-(N-acetyl-alpha-neuraminosyl-(2->3))-O-beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranosyl)-ceramide.,function:Involved in the biosynthesis of gangliosides GM2, GD2 and GA2.,online information:Beta-1,4 N-acetylgalactosaminyltransferase 1,online information:GlycoGene database,pathway:Protein modification; protein glycosylation.,similarity:Belongs to the glycosyltransferase 2 family.,subunit:Homodimer; disulfide-linked.,
Subcellular Location:Golgi apparatus membrane ; Single-pass type II membrane protein .
Expression: Brain,