Target：IFI-16

Fields：NOD-like receptor signaling pathway

Gene Name：IFI16

Protein Name：Gamma-interferon-inducible protein 16

Human Gene Id：3428

Human Swiss Prot No：Q16666

Immunogen：The antiserum was produced against synthesized peptide derived from human IFI16. AA range:731-780

Specificity：IFI-16 Polyclonal Antibody detects endogenous levels of IFI-16 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. ELISA: 1:40000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：IFI16;IFNGIP1;Gamma-interferon-inducible protein 16;Ifi-16;Interferon-inducible myeloid differentiation transcriptional activator

Observed Band(KD)：88kD

Background： This gene encodes a member of the HIN-200 (hematopoietic interferon-inducible nuclear antigens with 200 amino acid repeats) family of cytokines. The encoded protein contains domains involved in DNA binding, transcriptional regulation, and protein-protein interactions. The protein localizes to the nucleoplasm and nucleoli, and interacts with p53 and retinoblastoma-1. It modulates p53 function, and inhibits cell growth in the Ras/Raf signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2011],

Function：function:May function as a transcriptional repressor. Could have a role in the regulation of hematopoeitic differentiation through activation of unknown target genes. Controls cellular proliferation by modulating the functions of cell cycle regulatory factors including p53/TP53 and the retinoblastoma protein. May be involved in the senescence of prostate epithelial cells.,induction:Strongly induced by gamma interferon and, to a lesser extent, by alpha interferon. In HL-60 cells, maximum induction by gamma-interferon occurs within 12 hours whereas, for alpha-interferon, only 10-fold induction was observed after 36 hours. Induced in vitro by dimethylsulfoxide, retinoic acid and 1,25 dihydroxyvitamin D3.,PTM:Isoform 3 seems to show a minor degree of complex carbohydrate addition.,PTM:Phosphorylated on Ser and Thr.,sequence caution:Intron retention.,similarity:Belongs to the HIN-200 family.,

Subcellular Location：Nucleus. Cytoplasm. Cellular distribution is dependent on the acetylation status of the multipartite nuclear localization signal (NLS); NLS acetylation promotes cytoplasmic localization. Localizes in the nucleus during human herpes simplex virus 1 (HHV-1) infection.

Expression：Expressed in peripheral blood leukocytes, fibroblasts and lymphoid cells. Present in myeloid precursors (CD34+) and throughout monocyte development, but its expression is down-regulated in erythroid and polymorphonuclear precursor cells. Present in prostate, ovary and breast (at protein level).