Target：IARS2

Fields：Aminoacyl-tRNA biosynthesis

Gene Name：IARS2

Protein Name：Isoleucine--tRNA ligase mitochondrial

Human Gene Id：55699

Human Swiss Prot No：Q9NSE4

Mouse Gene Id：381314

Mouse Swiss Prot No：Q8BIJ6

Immunogen：The antiserum was produced against synthesized peptide derived from human IARS2. AA range:71-120

Specificity：IARS2 Polyclonal Antibody detects endogenous levels of IARS2 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：IARS2;Isoleucine--tRNA ligase; mitochondrial;Isoleucyl-tRNA synthetase;IleRS

Observed Band(KD)：114kD

Background： Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of isoleucine-tRNA synthetase exist, a cytoplasmic form and a mitochondrial form. This gene encodes the mitochondrial isoleucine-tRNA synthetase which belongs to the class-I aminoacyl-tRNA synthetase family. [provided by RefSeq, Dec 2014],

Function：catalytic activity:ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).,similarity:Belongs to the class-I aminoacyl-tRNA synthetase family.,

Subcellular Location：Mitochondrion matrix .

Expression： Brain,Epithelium,Liver,Placenta,Skin,