Target:ST5
Gene Name:ST5
Protein Name:Suppression of tumorigenicity 5 protein
Human Gene Id:6764
Human Swiss Prot No:P78524
Mouse Gene Id:76954
Mouse Swiss Prot No:Q924W7
Immunogen:The antiserum was produced against synthesized peptide derived from human ST5. AA range:411-460
Specificity:ST5 Polyclonal Antibody detects endogenous levels of ST5 protein.
Formulation:Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source:Polyclonal, Rabbit,IgG
Dilution:WB 1:500 - 1:2000. ELISA: 1:20000. Not yet tested in other applications.
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:1 mg/ml
Storage Stability:-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name:ST5;DENND2B;HTS1;Suppression of tumorigenicity 5 protein;DENN domain-containing protein 2B;HeLa tumor suppression 1
Observed Band(KD):126kD
Background: This gene was identified by its ability to suppress the tumorigenicity of Hela cells in nude mice. The protein encoded by this gene contains a C-terminal region that shares similarity with the Rab 3 family of small GTP binding proteins. This protein preferentially binds to the SH3 domain of c-Abl kinase, and acts as a regulator of MAPK1/ERK2 kinase, which may contribute to its ability to reduce the tumorigenic phenotype in cells. Three alternatively spliced transcript variants of this gene encoding distinct isoforms are identified. [provided by RefSeq, Jul 2008],
Function:function:May be involved in cytoskeletal organization and tumorgenicity. Isoform 1 seems to be involved in a signaling transduction pathway leading to activation of MAPK1/ERK2. Isoform 3 may block ERK2 activation stimulated by ABL1. Isoform 3 may alter cell morphology and cell growth.,similarity:Contains 1 dDENN domain.,similarity:Contains 1 DENN domain.,similarity:Contains 1 uDENN domain.,subunit:Isoform 1 interacts with the SH3 domain of ABL1.,tissue specificity:Widely expressed with the exception of peripheral blood lymphocytes. Isoform 1 is expressed in several epithelial and fibroblast (including tumorigenic) but absent in lymphoid cell lines (at protein level). Isoform 3 is expressed in primary cell or weakly tumorigenic but not in tumorigenic cell lines (at protein level).,
Subcellular Location:[Isoform 1]: Cytoplasm, cell cortex . Cell membrane . Recycling endosome . Colocalizes with RAB13 and ITSN1 at cytoplasmic vesicles that are most likely recycling endosomes. Colocalizes with the cortical actin cytoskeleton. .
Expression:Widely expressed with the exception of peripheral blood lymphocytes. Isoform 1 is expressed in several epithelial and fibroblast (including tumorigenic) but absent in lymphoid cell lines (at protein level). Isoform 3 is expressed in primary cell or weakly tumorigenic but not in tumorigenic cell lines (at protein level).