Target：APS

Fields：Neurotrophin signaling pathway;Insulin signaling pathway

Gene Name：SH2B2

Protein Name：SH2B adapter protein 2

Human Gene Id：10603

Human Swiss Prot No：O14492

Mouse Gene Id：23921

Mouse Swiss Prot No：Q9JID9

Rat Gene Id：114203

Rat Swiss Prot No：Q9Z200

Immunogen：Synthesized peptide derived from the Internal region of human APS.

Specificity：APS Polyclonal Antibody detects endogenous levels of APS protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. ELISA: 1:20000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：SH2B2;APS;SH2B adapter protein 2;Adapter protein with pleckstrin homology and Src homology 2 domains;SH2 and PH domain-containing adapter protein APS

Observed Band(KD)：67kD

Background： The protein encoded by this gene is expressed in B lymphocytes and contains pleckstrin homology and src homology 2 (SH2) domains. In Burkitt's lymphoma cell lines, it is tyrosine-phosphorylated in response to B cell receptor stimulation. Because it binds Shc independent of stimulation and Grb2 after stimulation, it appears to play a role in signal transduction from the receptor to the Shc/Grb2 pathway. [provided by RefSeq, Jun 2009],

Function：function:Adapter protein for several members of the tyrosine kinase receptor family. Involved in multiple signaling pathways. May be involved in coupling from immunoreceptor to Ras signaling. Acts as a negative regulator of cytokine signaling in collaboration with CBL. Binds to EPOR and suppresses EPO-induced STAT5 activation, possibly through a masking effect on STAT5 docking sites in EPOR. Suppresses PDGF-induced mitogenesis. May induce cytoskeletal reorganization via interaction with VAV3.,PTM:Tyrosine phosphorylated by JAK2, KIT and other kinases activated by B-cell receptor in response to stimulation with cytokines, IL3, IL5, PDGF, IGF1, IGF2, CSF2/GM-CSF and cross-linking of the B-cell receptor complex.,similarity:Belongs to the SH2B adapter family.,similarity:Contains 1 PH domain.,similarity:Contains 1 SH2 domain.,subcellular location:Cytoplasmic before PDGF stimulation. After PDG

Subcellular Location：Cytoplasm . Cell membrane . Cytoplasmic before PDGF stimulation. After PDGF stimulation, localized at the cell membrane and peripheral region.

Expression：Expressed in spleen, prostate, testis, uterus, small intestine and skeletal muscle. Among hematopoietic cell lines, expressed exclusively in B-cells. Not expressed in most tumor cell lines.